Health Lab

Test Name:

VITAMIN B7 (BIOTIN)

Test Code:

2199573

Alias:

LAB9065

CPT Code(s):

84591

Preferred Specimen:

2 mL frozen serum - protect from light

Container:

SST (gold)

Minimum Volume:

1 mL serum - protect from light

Collection Instructions:

Allow sample to clot for 30 minutes, spin at 3000 rpm for 10 minutes and transfer serum to a plastic amber vial. If amber vial is not available, wrap tube in aluminum foil to protect from light. Freeze within 30 minutes and send frozen.

Transport Temperature:

Frozen

Stability:

Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 14 days

Rejection Criteria:

Gross hemolysis • Lipemia • Received room temperature • Received refrigerated • Samples not protected from light • Samples other than serum

Additional Information:

The performance characteristics of the listed assay was validated by Cambridge Biomedical Inc. The US FDA has not approved or cleared this test. The results of this assay can be used for clinical diagnosis without FDA approval.
Cambridge Biomedical Inc. is a CLIA certified, CAP accredited laboratory for performing high complexity assays such as this one.

Schedule:

Set up: Varies; Report available: 5-7 days

Method:

Bioassay (BA)

Performing Lab:

Quest Diagnostics, Wood Dale #391 >Cambridge Biomedical Research Group, Boston, MA

Clinical Significance:

Biotin (Vitamin B7) - Biotin, vitamin B7, or vitamin H, is a water soluble vitamin. The vitamin plays a role in the transferring of carbon dioxide in the metabolism of fat, carbohydrate and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone by the action of biotinyl-transferase. Sometimes the vitamin is used in weight reduction programs. It may be prescribed as a supplement for diabetic patients due to its role in carbohydrate metabolism. Biotin is commonly found in vitamin B complex and many food sources, such as milk, yeast, egg yolk, cereal, and mushrooms. The reference daily intake [RDI of 101.9(c) (8) (IV)] for vitamin B7 is 300 micrograms. Deficiency in the vitamin may result in seborrheic dermatitis, alopecia, myalgia, hyperesthesia, and conjunctivitis. Disorders of biotin metabolism can be acquired or congenital. Biotinidase and holocarboxylase synthethase deficiency are the two better known forms of disorders. The lack of biotin-dependent pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and acetyl-CoA carboxylase can lead to the life-threatening disorder of multiple carboxylase deficiency. Treatment involves a daily dose of approximately 10 mg biotin/day. Irreversible mental or neurological abnormalities may result from delayed clinical intervention.

Use:

Updated: 08/15/2025

Reference Ranges:

<12 years	100.0-2460.2 pg/mL
≥12 years	221.0-3004.0 pg/mL

The CPT codes included in this publication are in accordance with Current Procedural Terminology, a publication of the American Medical Association. CPT codes are provided here for the convenience of our clients; however, correct coding often varies from one carrier to another, and HealthLab may bill specific carriers using codes other than those shown. Clients who bill for services should verify the code(s) with the applicable payor to confirm that their use is appropriate in each case.